Combinatorial Chemistry

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Honggu Chun et al, “Bio-cell Chip Fabrication and Applications,” in Microchip Methods in Diagnostics, Series: Methods in Molecular Biology (vol.509), The Humana Press, 145-158, ISBN: 978-1-58829-955-0 (2009.02)

Most biological reactions occur based on multiple substrates. But conventional methods are not suitable to quantify reaction kinetics, because numerous repetitive experiments lead to unwanted time delay between experiments, and large amount of reagents are needed to repeat similar experiments. PDMS microfluidic chip can be a good tool for this use. It has numerous merits such as, biocompatibility, ease of fabrication, optical transparency, and oxygen permeability. In this study, multi-layer PDMS microfluidic chip was suggested which is capable of generating 36 concentration gradients of two substrates. Using this, reaction kinetics of various biological experiments can be analyzed by single chip experiments